Gelsolin antibody (AA 432-553)

Catalog No. ABIN1078060

This Rabbit Polyclonal antibody specifically detects Gelsolin in WB, IHC, IP and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Gelsolin antibody (AA 432-553) (ABIN1078060)

Target: Gelsolin (GSN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Gelsolin Antibody

Binding Specificity: AA 432-553

Purpose: Polyclonal Antibody to Gelsolin (GSN)

Specificity: The antibody is a rabbit polyclonal antibody raised against GSN. It has been selected for its ability to recognize GSN in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA372Hu01Recombinant Gelsolin (GSN)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Gelsolin antibody (ABIN1078060)

Hergenroeder, Redell, Moore, Dubinsky, Funk, Crommett, Clifton, Levine, Valadka, Dash: "Identification of serum biomarkers in brain-injured adults: potential for predicting elevated intracranial pressure." in: Journal of neurotrauma, Vol. 25, Issue 2, pp. 79-93, (2008) (PubMed).

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: Gelsolin

Background: GS, AGEL, ADF, Brevin, Gelsolin Amyloidosis,Finnish Type, Actin-depolymerizing factor

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy