Cellular Fibronectin antibody

Catalog No. ABIN108413

The Mouse Monoclonal anti-Cellular Fibronectin antibody has been validated for IHC, WB and ELISA. It is suitable to detect Cellular Fibronectin in samples from Human, Chicken, Guinea Pig, Rabbit and Rat.

Quick Overview for Cellular Fibronectin antibody (ABIN108413)

Target: Cellular Fibronectin (Fibronectin, Cellular)

Reactivity: Human, Chicken, Guinea Pig, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cellular Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: DH1

Product Details anti-Cellular Fibronectin Antibody

Specificity: The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin. Mab DHI has been shown to specifically block chondrocyte condensation in chicken embryos (5). The antibody crossreacts with human, chicken, rat, rabbit and guinea pig

Immunogen: Monoclonal antibody (Mab) to cFn is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified fibronectin from A8387 fibrosarcoma cells was used as an immunogen (21).

Isotype: IgG1

Application Details

Application Notes: No reactivity with paraffin sections. Working dilution in western blotting is at least 1:1000.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Cellular Fibronectin

Target: Cellular Fibronectin (Fibronectin, Cellular)

Alternative Name: Cellular Fibronectin (cFn)

Background: The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas.