CRD1 antibody (AA 1-409)

Catalog No. ABIN108469

This Rabbit Polyclonal antibody specifically detects CRD1 in WB. It exhibits reactivity toward Bacteria (Congregibacter), Bacteria (Roseobacter), Chlamydomonas reinhardtii, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum and Pisum sativum and has been mentioned in 3+ publications.

Quick Overview for CRD1 antibody (AA 1-409) (ABIN108469)

Target: CRD1 (CORD1) (Cone Rod Dystrophy 1 (Autosomal Dominant) (CORD1))

Reactivity: Bacteria (Congregibacter), Bacteria (Roseobacter), Chlamydomonas reinhardtii, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum, Pisum sativum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRD1 Antibody

Binding Specificity: AA 1-409

Characteristics: Expected / apparent Molecular Weight of the Antigene: 47 / 40 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA Q9M591, At3g56940

Application Details

Application Notes: Recommended Dilution: 1 : 3000 with alkaline phosphatase (WB).

Comment:

Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-CRD1 antibody (ABIN108469)

Boldareva-Nuianzina, Bláhová, Sobotka, Koblízek: "Distribution and origin of oxygen-dependent and oxygen-independent forms of Mg-protoporphyrin monomethylester cyclase among phototrophic proteobacteria." in: Applied and environmental microbiology, Vol. 79, Issue 8, pp. 2596-604, (2013) (PubMed).

Hollingshead, Kopecná, Jackson, Canniffe, Davison, Dickman, Sobotka, Hunter: "Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803." in: The Journal of biological chemistry, Vol. 287, Issue 33, pp. 27823-33, (2012) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Target Details for CRD1

Target: CRD1 (CORD1) (Cone Rod Dystrophy 1 (Autosomal Dominant) (CORD1))

Alternative Name: CRD1

Background: AGI Code: At3g56940
Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

Molecular Weight: expected: 47 kDa, apparent: 40 kDa (Arabidopsis thaliana)

UniProt: Q9M591