CRD1 antibody (AA 1-409)

Catalog No. ABIN108469

This Rabbit Polyclonal antibody specifically detects CRD1 in WB. It exhibits reactivity toward Arabidopsis thaliana, Bacteria (Roseobacter), Chlamydomonas reinhardtii, Chloroflexus aurantiacus, Congregibacter litoralis, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum, Pea, Physcomytrium Patens, Rubrivivax gelatinosus and Synechocystis. It has been mentioned in 3+ publications

Quick Overview for CRD1 antibody (AA 1-409) (ABIN108469)

Target: CRD1 (CORD1) (Cone Rod Dystrophy 1 (Autosomal Dominant) (CORD1))

Reactivity: Arabidopsis thaliana, Bacteria (Roseobacter), Chlamydomonas reinhardtii, Chloroflexus aurantiacus, Congregibacter litoralis, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum, Pea, Physcomytrium Patens, Rubrivivax gelatinosus, Synechocystis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRD1 Antibody

Binding Specificity: AA 1-409

Purpose: Anti-CRD1 | Cyanobacterial homolog of plant CHL27 cyclase

Cross-Reactivity (Details): Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, Physcomitrium patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27), Synechocystis PCC 6803, anoxygenic phototrophs (proeobacteria): Congregibacter litoralis, Roseobacter litoralis, green non-sulfur bacterium: Chloroflexus aurantiacus, photosynthetic bacterium: Rubriviavax gelatinosus
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Cyanobacteria,Gossyium hirsutum,Euphorbia esula,Hordeum vulgare,Nannochloropsis gaditana,Ricinus communis

Purification: Serum

Immunogen: residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591

Application Details

Application Notes: 1 : 3000 (WB)

Comment:

Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-CRD1 antibody (ABIN108469)

Boldareva-Nuianzina, Bláhová, Sobotka, Koblízek: "Distribution and origin of oxygen-dependent and oxygen-independent forms of Mg-protoporphyrin monomethylester cyclase among phototrophic proteobacteria." in: Applied and environmental microbiology, Vol. 79, Issue 8, pp. 2596-604, (2013) (PubMed).

Hollingshead, Kopecná, Jackson, Canniffe, Davison, Dickman, Sobotka, Hunter: "Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803." in: The Journal of biological chemistry, Vol. 287, Issue 33, pp. 27823-33, (2012) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Target Details for CRD1

Target: CRD1 (CORD1) (Cone Rod Dystrophy 1 (Autosomal Dominant) (CORD1))

Alternative Name: CRD1

Background:

Background: Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

Additional information: [compartment marker] of chloroplast thylakoid and envelope membranes

Molecular Weight: 47 | 40 kDa (Arabidopsis thaliana)

Gene ID: 824861

UniProt: Q9M591