BUB1 antibody (AA 281-419)

Catalog No. ABIN108554

The Mouse Monoclonal anti-BUB1 antibody has been validated for WB, IF, IHC (p), ICC and FACS. It is suitable to detect BUB1 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for BUB1 antibody (AA 281-419) (ABIN108554)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 14H5

Product Details anti-BUB1 Antibody

Binding Specificity: AA 281-419

Cross-Reactivity: Kangaroo, Rat, Xenopus laevis

Cross-Reactivity (Details): Mouse, Human. Predicted to work with: Rat, Xenopus laevis, Kangaroo

Purification: Protein A purified

Immunogen: Fusion protein, corresponding to amino acids 281-419 of Human Bub 1

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try 1g/mL final concentration

Comment:

Myeloma, fusion partners: SP2/0,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-BUB1 antibody (ABIN108554)

Boyarchuk, Salic, Dasso, Arnaoutov: "Bub1 is essential for assembly of the functional inner centromere." in: The Journal of cell biology, Vol. 176, Issue 7, pp. 919-28, (2007) (PubMed).

Kittler, Pelletier, Heninger, Slabicki, Theis, Miroslaw, Poser, Lawo, Grabner, Kozak, Wagner, Surendranath, Richter, Bowen, Jackson, Habermann, Hyman, Buchholz: "Genome-scale RNAi profiling of cell division in human tissue culture cells." in: Nature cell biology, Vol. 9, Issue 12, pp. 1401-12, (2007) (PubMed).

Qi, Tang, Yu: "Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1." in: Molecular biology of the cell, Vol. 17, Issue 8, pp. 3705-16, (2006) (PubMed).

Stavropoulou, Vasquez, Cereser, Freda, Masucci: "TPPII promotes genetic instability by allowing the escape from apoptosis of cells with activated mitotic checkpoints." in: Biochemical and biophysical research communications, Vol. 346, Issue 2, pp. 415-25, (2006) (PubMed).

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: Bub1

Background: Synonyms:
BUB1 budding uninhibited by benzimidazoles 1 homolog antibody,BUB1 budding uninhibited by benzimidazoles 1homolog (yeast) antibody,BUB1A antibody,BUB1L antibody,Budding uninhibited by benzimidazoles 1 (yeast homolog)antibody,Budding uninhibited by benzimidazoles 1 homolog antibody,hBUB1 antibody,Mitotic checkpointserine/threonine protein kinase BUB1 antibody,Mitotic spindle checkpoint kinase antibody,Putativeserine/threonine protein kinase antibody

Gene ID: 699, 12235, 296137

OMIM: 602452

UniProt: O43683, O08901