HNRNPL antibody

Catalog No. ABIN108560

Cited in 4+ publications. The Mouse Monoclonal anti-HNRNPL antibody (Clone 1G11) (ABIN108560) specifically detects HNRNPL in WB, ELISA, IF, IHC (p), IP and ICC. The antibody is reactive with Human, Mouse and Xenopus laevis samples.

Quick Overview for HNRNPL antibody (ABIN108560)

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Reactivity: Human, Mouse, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1G11

Product Details anti-HNRNPL Antibody

Specificity: Human hnRNP F/H

Purification: Protein A

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try:IHC-P: Use a concentration of 1 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB: Use a concentration of 1 μg/mL. Detects a band of approximately 53 kDa (hnRNP F), 56 kDa (hnRNP H). (predicted molecular weight: hnRNP F = 49.8 kDa, hnRNP H = 53.9 kDa).

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPL antibody (ABIN108560)

Marzano, Santini, Rossi, Zucchelli, DAlessandro, Marchetti, Mingardi, Stagni, Barilà, Urbani: "Proteomic profiling of ATM kinase proficient and deficient cell lines upon blockage of proteasome activity." in: Journal of proteomics, Vol. 75, Issue 15, pp. 4632-46, (2012) (PubMed).

Tsai, Wang, Huang, Waterman: "Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 4, pp. M110.007385, (2011) (PubMed).

Millevoi, Decorsière, Loulergue, Iacovoni, Bernat, Antoniou, Vagner: "A physical and functional link between splicing factors promotes pre-mRNA 3' end processing." in: Nucleic acids research, Vol. 37, Issue 14, pp. 4672-83, (2009) (PubMed).

Paronetto, Achsel, Massiello, Chalfant, Sette: "The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x." in: The Journal of cell biology, Vol. 176, Issue 7, pp. 929-39, (2007) (PubMed).

Target Details for HNRNPL

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Alternative Name: hnRNP F+H 

Background: Synonyms:
Heterogeneous nuclear ribonucleoprotein F antibody,Heterogeneous nuclear ribonucleoprotein H1antibody,hnRNP F antibody,hnRNP H antibody,hnRNPF antibody,hnRNPH antibody,HNRNPH1 antibody,HNRPFantibody,HNRPH antibody,HNRPH1 antibody,mcs94 1 antibody,Nucleolin like protein antibody,Nucleolin likeprotein mcs94 1 antibody

Gene ID: 3185, 3187

UniProt: P52597, P31943