GZMK antibody

Catalog No. ABIN108722

The Mouse Monoclonal anti-GZMK antibody is suitable to detect GZMK in samples from Human. It has been validated for ELISA, FACS and cELISA.

Quick Overview for GZMK antibody (ABIN108722)

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMK antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Cell-ELISA (cELISA)

Clone: GM-6C3

Product Details anti-GZMK Antibody

Specificity: The antibodies do not cross-react with the human granzymes A, B or M.

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding human Granzyme K

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

ELISA pair with GM26E7 (catcher)

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for GZMK

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Alternative Name: Granzyme K

Background: Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. GM24C3 was generated by genetic immunisation and reacts with human granzyme K (GrK), a 28 kDa serine protease with trypsin-like specificity.

UniProt: P49863