SPIB antibody (AA 200-252)

Catalog No. ABIN1098113

The Mouse Monoclonal anti-SPIB antibody has been validated for WB, ELISA, IHC, ICC and FACS. It is suitable to detect SPIB in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for SPIB antibody (AA 200-252) (ABIN1098113)

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4G5

Product Details anti-SPIB Antibody

Binding Specificity: AA 200-252

Purpose: SPIB Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SPIB antibody (ABIN1098113)

Marshall, Dunham, Major: "Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity." in: The Journal of general virology, Vol. 91, Issue Pt 12, pp. 3042-52, (2010) (PubMed).

Nagasawa, Schmidlin, Hazekamp, Schotte, Blom: "Development of human plasmacytoid dendritic cells depends on the combined action of the basic helix-loop-helix factor E2-2 and the Ets factor Spi-B." in: European journal of immunology, Vol. 38, Issue 9, pp. 2389-400, (2008) (PubMed).

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB

Background: The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 28.8 kDa

Gene ID: 6689

UniProt: Q01892