VGlut3 Transporter (AA 546-588), (C-Term) antibody

Catalog No. ABIN1098207

The Mouse Monoclonal anti-VGlut3 Transporter antibody (Clone S34-34) (ABIN1098207) specifically detects VGlut3 Transporter in IHC, WB and IF/ICC. The antibody is reactive with Rat samples.

Quick Overview for VGlut3 Transporter (AA 546-588), (C-Term) antibody (ABIN1098207)

Target: VGlut3 Transporter

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S34-34

Product Details

Binding Specificity: AA 546-588, C-Term

Purpose: Anti-VGlut3 Transporter Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat VGlut3. It does not cross-react with VGlut1 or VGlut2.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): No cross Reactivity with Vglut1 and Vglut2

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession no. NP_74947.1).

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of ~65 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: CV-1 fibroblasts transfected with VGlut3.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details

Target: VGlut3 Transporter

Background: Vesicular glutamate transporter 3,Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:12097496, PubMed:12388773}.,The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut3 is a multipass membrane protein restricted to synaptic vesicles of glutamatergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus.,Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Membrane, Cell junction, synapse, synaptosome,VGluT3, Solute carrier family 17 member 8

UniProt: Q7TSF2