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TPI1 antibody

This Sheep Polyclonal antibody specifically detects TPI1 in IP. It exhibits reactivity toward Rabbit.
Catalog No. ABIN1099705

Quick Overview for TPI1 antibody (ABIN1099705)

Target

See all TPI1 Antibodies
TPI1 (Triosephosphate Isomerase 1 (TPI1))

Reactivity

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Rabbit

Host

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Sheep

Clonality

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Polyclonal

Conjugate

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  • 1
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  • 1
This TPI1 antibody is un-conjugated

Application

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Immunoprecipitation (IP)
  • Specificity

    Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Characteristics

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

    Physicochemical characteristics: PAb concentration 1 mg/0.5ml. No foreign proteins added.

    Purification

    Antisera
    Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

    Immunogen

    Triosephosphate isomerase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes

    Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/100 and 1/1,000.

    Comment

    Physical form: Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.

    Preservative

    Without preservative

    Handling Advice

    The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

    Storage

    4 °C
  • Target

    TPI1 (Triosephosphate Isomerase 1 (TPI1))

    Alternative Name

    triosephosphate isomerase

    Background

    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.

    Pathways

    Cell RedoxHomeostasis
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