Trypsin antibody

Catalog No. ABIN1099706

The Rabbit Polyclonal anti-Trypsin antibody has been validated for WB, ELISA, IF and DB. It is suitable to detect Trypsin in samples from Cow.

Quick Overview for Trypsin antibody (ABIN1099706)

Target: Trypsin

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-Trypsin Antibody

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Physicochemical characteristics: IgG protein concentration 10 mg/ml. No foreign proteins added.

Purification: Antisera
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Trypsin isolated and purified from bovine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/7,000.

Comment:

Physical form: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.

Preservative: Without preservative

Handling Advice: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C

Target Details for Trypsin

Target: Trypsin

Background: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.