Serum antibody

Catalog No. ABIN1099711

This Rabbit Polyclonal antibody specifically detects Serum in IP. It exhibits reactivity toward Horse.

Quick Overview for Serum antibody (ABIN1099711)

Target: Serum

Reactivity: Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP)

Product Details

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homolo- gous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.

Characteristics: Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components. In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipi- tation can be observed of not less than 12 individual proteins components.

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Purification: Antisera
Adsorption: No adsorption required.

Immunogen: Pooled whole horse serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Comment:

Physical form: Delipidated, heat inactivated, lyophilized, stable whole antiserum.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Preservative: Without preservative

Handling Advice: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Storage: 4 °C

Target Details

Target: Serum

Alternative Name: Serum Proteins

Background: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immuno- precipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
Directions for use: In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.