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beta Actin antibody (N-Term)

This anti-beta Actin antibody is a Mouse Monoclonal antibody detecting beta Actin in WB, ELISA and IHC. Suitable for Human, Mouse, Rat, Rabbit and Pig.
Catalog No. ABIN1100220

Quick Overview for beta Actin antibody (N-Term) (ABIN1100220)

Target

See all beta Actin (ACTB) Antibodies
beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity

  • 197
  • 138
  • 117
  • 55
  • 45
  • 43
  • 40
  • 27
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  • 2
  • 2
  • 2
  • 1
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  • 1
  • 1
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  • 1
Human, Mouse, Rat, Rabbit, Pig

Host

  • 117
  • 102
  • 5
  • 1
  • 1
Mouse

Clonality

  • 112
  • 112
Monoclonal

Conjugate

  • 160
  • 11
  • 11
  • 9
  • 3
  • 3
  • 3
  • 3
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  • 2
  • 1
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  • 1
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  • 1
  • 1
  • 1
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  • 1
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  • 1
This beta Actin antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone

3H2-E4-E12
  • Binding Specificity

    • 41
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    • 3
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    • 1
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    • 1
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    • 1
    N-Term

    Specificity

    Beta Actin antibody is specific for human and mouse beta Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.

    Purification

    Protein A purified

    Sterility

    Sterile filtered

    Immunogen

    Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations withbeta-actin peptide near the N-Terminus followed by hybridoma development.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG2a
  • Application Notes

    Approved: ELISA, IHC, WB (1 μg/mL)

    Usage: Anti-beta Actin antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    avoid freeze thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Target

    beta Actin (ACTB) (Actin, beta (ACTB))

    Alternative Name

    ACTB / Beta Actin

    Background

    Name/Gene ID: ACTB

    Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

    Gene ID

    60

    UniProt

    P60709

    Pathways

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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