BMP7 antibody (AA 315-431)

Catalog No. ABIN1100288

The Rabbit Polyclonal anti-BMP7 antibody is suitable to detect BMP7 in samples from Human. It has been validated for WB, IHC, ELISA, IHC (p) and Neut.

Quick Overview for BMP7 antibody (AA 315-431) (ABIN1100288)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Product Details anti-BMP7 Antibody

Binding Specificity: AA 315-431

Specificity: Human BMP-7

Purification: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human BMP-7.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (2.5 μg/mL), Neut (0.4 - 0.6 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7431 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human BMP-7 (100 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: To detect hBMP-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-7 (LS-C104294) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP7 / BMP-7

Background: Name/Gene ID: BMP7
Family: TGF beta

Synonyms: BMP7, Bone morphogenetic protein 7, Eptotermin alfa, OP-1, OP1, Osteogenic protein 1, BMP-7

Gene ID: 655

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance