CTGF antibody

Catalog No. ABIN1101002

The Rabbit Polyclonal anti-CTGF antibody has been validated for WB, IHC, ELISA, IHC (p) and Neut. It is suitable to detect CTGF in samples from Human.

Quick Overview for CTGF antibody (ABIN1101002)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Product Details anti-CTGF Antibody

Specificity: Human CTGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human CTGF.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (6 - 9 μg/mL), WB

Usage: Immunohistochemistry: LS-B7479 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCTGF (5 μg/mL), a concentration of 6-9 μg/mL of this antibody is required. ELISA: To detect hCTGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (LS-C104563) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF

Background: Name/Gene ID: CTGF
Family: Hormone

Synonyms: CTGF, CCN family member 2, CCN2, IGFBP8, IGF-binding protein 8, NOV2, HCS24, IBP-8, IGFBP-8

Gene ID: 1490

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding