alpha Defensin 1 antibody

Catalog No. ABIN1101107

The Goat Polyclonal anti-alpha Defensin 1 antibody has been validated for WB, IHC, ELISA, Neut and IHC (p). It is suitable to detect alpha Defensin 1 in samples from Human.

Quick Overview for alpha Defensin 1 antibody (ABIN1101107)

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Defensin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha Defensin 1 Antibody

Brand: IHC-plus™

Specificity: Human NP-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NP-1.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.4 - 0.6 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7416 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human NP-1 (100 ng/mL), a concentration of 0.40-0.60 μg/mL of this antibody is required. ELISA: To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NP-1 (LS-C104516) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: DEFA1 / HP1

Background: Name/Gene ID: DEFA1

Synonyms: DEFA1, DEFA2, Defensin, alpha 2, HP-1, HP1, Myeloid-related sequence, MRS, DEF1, Defensin, alpha 1, HNP-1, Neutrophil defensin 1

Gene ID: 1667

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin