HSP70 antibody

Catalog No. ABIN1102000

This Mouse Monoclonal antibody specifically detects HSP70 in WB, IHC, IF, IP, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Chicken, Drosophila melanogaster, Fish, Saccharomyces cerevisiae and Amphibian.

Quick Overview for HSP70 antibody (ABIN1102000)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Mouse, Rat, Chicken, Drosophila melanogaster, Fish, Saccharomyces cerevisiae, Amphibian

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5A5

Product Details anti-HSP70 Antibody

Brand: IHC-plus™

Specificity: Detects several members of the heat shock protein 70kD gene family including Hsp70, Hsc70, Grp78, and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.

Purification: Protein G purified from tissue culture supernatant

Immunogen: Human Recombinant Hsp70 overexpressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (1:500), IF (1:500), IHC, IHC-P (10 μg/mL), IP, WB (1:1000)

Usage: Western blot performed with lysates from rat skeletal muscle, brain, heart, kidney, lung, pancreas, spleen, testes, thymus.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 / Heat Shock Protein 70

Background: Name/Gene ID: HSP70

Synonyms: HSP70