IL-22 antibody

Catalog No. ABIN1102439

The Rabbit Polyclonal anti-IL-22 antibody has been validated for WB, ELISA, IHC, Neut and IHC (p). It is suitable to detect IL-22 in samples from Human.

Quick Overview for IL-22 antibody (ABIN1102439)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL-22 Antibody

Brand: IHC-plus™

Specificity: Human IL-22

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-22.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (1.25 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7437 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-22 (50 ng/mL), a concentration of 1.25 μg/mL of this antibody is required. ELISA: To detect hIL-22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-22 (LS-C104319) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-22. Western Blot: To detect hIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL22

Background: Name/Gene ID: IL22
Family: Interleukin

Synonyms: IL22, Cytokine Zcyto18, ILTIF, Interleukin 22, IL-22, IL-D110, TIFIL-23, Zcyto18, IL-TIF, Interleukin-22

Gene ID: 50616

UniProt: Q9GZX6