CCL2 antibody

Catalog No. ABIN1102997

The Rabbit Polyclonal anti-CCL2 antibody is suitable to detect CCL2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CCL2 antibody (ABIN1102997)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL2 Antibody

Specificity: This antibody is specific for human MCP-1 protein. A BLAST analysis was used to suggest cross-reactivity with MCP-1 from human sources based on 100 % homology with the immunizing sequence. Based on 98 to 100 % homology, there is a chance of cross-reactivity to MCP-1 from chimpanzee, orangutan, macaque, and baboon. Cross-reactivity with MCP-1 from other sources has not been determined.

Purification: Ion exchange chromatography

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11025 Da in size corresponding to the mature human MCP-1 protein by western blotting in appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: CCL2 / MCP-1

Background: Name/Gene ID: CCL2
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF

Gene ID: 6347

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection