PDGF-AA Homodimer antibody

Catalog No. ABIN1103534

The Rabbit Polyclonal anti-PDGF-AA Homodimer antibody is suitable to detect PDGF-AA Homodimer in samples from Human. It has been validated for WB, IHC, ELISA, IHC (p) and Neut.

Quick Overview for PDGF-AA Homodimer antibody (ABIN1103534)

Target: PDGF-AA Homodimer

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Product Details

Specificity: Human PDGF-AA

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PDGF-AA.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (5 - 7 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7488 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-AA (5 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-AA (LS-C104676) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details

Target: PDGF-AA Homodimer

Alternative Name: PDGF-AA

Background: Name/Gene ID: PDGFA
Family: PDGF

Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1

Gene ID: 5154

UniProt: P04085