RPE antibody (Biotin)

Catalog No. ABIN1104023

The Goat Polyclonal anti-RPE antibody has been validated for ELISA, WB and IHC. It is suitable to detect RPE in samples from Red algae (Gracilaria).

Quick Overview for RPE antibody (Biotin) (ABIN1104023)

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Reactivity: Red algae (Gracilaria)

Host: Goat

Clonality: Polyclonal

Conjugate: This RPE antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RPE Antibody (Biotin)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

Purification: Immunoaffinity purified

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC (1:500 - 1:3000), WB (1:2000 - 1:20000)

Usage: Suitable for immunoblotting (western or dot blot), ELISA as well as other immunochemical assays. R-Phycoerythrin (240 kD) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Gracilaria

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RPE

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Alternative Name: RPE

Background: Name/Gene ID: RPE

Synonyms: RPE, Ribulose-phosphate 3-epimerase, RPE2-1

Gene ID: 6120

UniProt: Q96AT9