VEGFA antibody

Catalog No. ABIN1104977

The Rabbit Polyclonal anti-VEGFA antibody has been validated for WB and ELISA. It is suitable to detect VEGFA in samples from Cow.

Quick Overview for VEGFA antibody (ABIN1104977)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VEGFA Antibody

Specificity: This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100 % homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95 % homology, and to rat and mouse based on 93 % homology. Cross-reactivity with VEGF-A from other sources has not been determined.

Purification: Protein A purified

Immunogen: Recombinant protein produced in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:333)

Usage: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kD in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA / VEGF

Background: Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling