ZIC2 antibody (AA 520-531)

Catalog No. ABIN1105113

This anti-ZIC2 antibody is a Goat Polyclonal antibody detecting ZIC2 in WB and ELISA. Suitable for Human, Rat, Mouse, Guinea Pig, Monkey and Bat.

Quick Overview for ZIC2 antibody (AA 520-531) (ABIN1105113)

Target: ZIC2

Reactivity: Human, Rat, Mouse, Guinea Pig, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZIC2 Antibody

Binding Specificity: AA 520-531

Specificity: Human ZIC2. This antibody is expected to NOT cross-react with other ZIC proteins.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Elephant, Opossum, Guinea pig (100%) Galago, Tamarin, Bovine, Dog, Hamster, Panda, Porcine, Xenopus, Medaka, Seabass, Pufferfish, Zebrafish, Stickleback (92%) Zebra finch, Chicken, Lizard, Salmon (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HSGLSSNFNEWY from the C-terminus of human ZIC2 (NP_009060.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Elephant, Opossum, Guinea pig (100%), Galago, Tamarin, Bovine, Dog, Hamster, Panda, Porcine, Xenopus, Medaka, Seabass, Pufferfish, Zebrafish, Stickleback (92%), Zebra finch, Chicken, Lizard, Salmon (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:1000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kD is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kD according to NP_009060.2 and 58.6kD for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman, Clark Atlanta University, GA, USA. We have observed an approx 75kD band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZIC2

Target: ZIC2

Alternative Name: ZIC2

Background: Name/Gene ID: ZIC2

Synonyms: ZIC2, HPE5, Zic family member 2, Zinc finger protein ZIC 2

Gene ID: 7546

NCBI Accession: NP_009060

UniProt: O95409

Pathways: Tube Formation