ACTN1 antibody

Catalog No. ABIN1105322

The Mouse Monoclonal anti-ACTN1 antibody has been validated for WB, IF, IHC (p) and IP. It is suitable to detect ACTN1 in samples from Human, Mouse, Rat and Rabbit.

Quick Overview for ACTN1 antibody (ABIN1105322)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: SA-20

Product Details anti-ACTN1 Antibody

Specificity: This antibody reacts to Alpha-actinin-1.

Purification: Affinity chromatography

Immunogen: Rabbit skeletal alpha-actinin

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % sodium acetate or neutral PBS

Concentration: 0,1 mg/mL

Buffer: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: alpha-Actinin-1 / ACTN1

Background: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.Synonyms: Alpha actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin 1

Gene ID: 87

NCBI Accession: NP_001093

UniProt: P12814

Pathways: Cell-Cell Junction Organization