AOC3 antibody

Catalog No. ABIN1105387

This Rat Monoclonal antibody specifically detects AOC3 in IF, IP, IHC (fro) and Func. It exhibits reactivity toward Mouse.

Quick Overview for AOC3 antibody (ABIN1105387)

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This AOC3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 01-07-88

Product Details anti-AOC3 Antibody

Specificity: The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It inhibits migration of granulocytes and monocytes in acute models of inflammation.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography

Immunogen: Vessels from mouse lymph nodes

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Alternative Name: AOC3 / VAP1

Background: VAP-1 is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.Synonyms: Copper amine oxidase, HPAO, Membrane primary amine oxidase, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1

Gene ID: 11754

NCBI Accession: NP_033805

UniProt: O70423

Pathways: Feeding Behaviour