Biotin antibody (FITC)

Catalog No. ABIN1105535

The Goat Polyclonal anti-Biotin antibody has been validated for FACS and IF. It is suitable to detect Biotin in samples from .

Quick Overview for Biotin antibody (FITC) (ABIN1105535)

Target: Biotin

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Biotin Antibody (FITC)

Characteristics: Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 4.6 Moles FITC per mole of Goat IgG.

Purification: Immunoaffinity chromatography.

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.