BPI antibody

Catalog No. ABIN1105557

This anti-BPI antibody is a Rabbit Polyclonal antibody detecting BPI in IP and EIA. Suitable for Human.

Quick Overview for BPI antibody (ABIN1105557)

Target: BPI (Bactericidal/Permeability Increasing Protein (BPI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BPI antibody is un-conjugated

Application: Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-BPI Antibody

Specificity: The polyclonal antibody reacts specifically with full length human natural and recombinant Bactericidal Permeability Increasing protein (BPI). It recognizes only free BPI and does not interact with BPI that has formed a complex with LPS.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for BPI

Target: BPI (Bactericidal/Permeability Increasing Protein (BPI))

Alternative Name: BPI

Background: The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin.Synonyms: Bactericidal permeability-increasing protein, CAP57

Gene ID: 671

NCBI Accession: NP_001716

UniProt: P17213