CD40 Ligand antibody (N-Term)

Catalog No. ABIN1105902

This anti-CD40 Ligand antibody is a Rabbit Polyclonal antibody detecting CD40 Ligand in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for CD40 Ligand antibody (N-Term) (ABIN1105902)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD40 Ligand Antibody

Binding Specificity: N-Term

Specificity: This antibody detects CD154 / CD40L at N-term. No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human CD40L .Remarks: Sequence is different from the relative sequence of mouse by two amino acids.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 50 % glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L

Background: CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints