Complex II Subunit 70 KDa Fp (C-Term) antibody

Catalog No. ABIN1106817

This anti- antibody is a Rabbit Polyclonal antibody detecting in IHC (p) and WB. Suitable for Human, Mouse and Rat.

Quick Overview for Complex II Subunit 70 KDa Fp (C-Term) antibody (ABIN1106817)

Target: Complex II Subunit 70 KDa Fp

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Specificity: This antibody detects Complex II subunit 70 kDa Fp (C-term). No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human, Rat.

Purification: Immunoaffinity Chromatography.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of Human SDHA .Remarks: Sequence is identical to the related Rat and Mouse sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % Sodium Acetate or Neutral PBS. If 0.5 mL of PBS is added the antibody concentration will be 0.1 mg/mL.

Buffer: 50 % Glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody after reconstitution at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Complex II Subunit 70 KDa Fp

Background: Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits, it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.Synonyms: Mitochondria Complex II (Succinat Dehydrogenase) 70 kD Fp subunit, SDH2, SDHA, SDHF, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene ID: 6389

NCBI Accession: NP_004159

UniProt: P31040