Cytokeratin 18 antibody

Catalog No. ABIN1106918

Product Details anti-Cytokeratin 18 Antibody

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Dog, Pig, Hamster, Rabbit, Zebrafish (Danio rerio), Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithel cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

Purification: Purified

Immunogen: Cytoskeletal preparation of cells.

Clone: RGE53

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2-8 °C. After reconstitution store in aliquots at -20 °C. The addition of sodium azide at 0.1% (w/v) is recommended for storage up to one month at 2-8° C.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18 (KRT18 Products)

Background: Cytokeratins are a subfamily of intermediatefilament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gene ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis