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Dystroglycan antibody

This anti-Dystroglycan antibody is a Mouse Monoclonal antibody detecting Dystroglycan in WB, IHC (p) and EIA. Suitable for Human, Mouse, Rat, Cow and Rabbit.
Catalog No. ABIN1107039

Quick Overview for Dystroglycan antibody (ABIN1107039)

Target

See all Dystroglycan (DAG1) Antibodies
Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity

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Human, Mouse, Rat, Cow, Rabbit

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Dystroglycan antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone

2238
  • Specificity

    This antibody is specific for a glycoepitope on brain bovine alpha-dystroglycan, which is absent on alpha-dystroglycan expressed in all other tissues.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human, mouse, rat, rabbit, bovine

    Purification

    Protein G purified

    Isotype

    IgG2b
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store at 2 - 8 °C.
  • Target

    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

    Alternative Name

    Dystroglycan

    Background

    Alpha-dystroglycan (alpha-DG), also known as dystrophin-associated glycoprotein, is a laminin-binding protein of ~156 kDa (including glyco-groups). Alpha-DG is a component of the dystroglycan complex, which is involved in early development, morphogenesis and in the pathogenesis of muscular dystrophies. Alpha- and beta-DG are encoded by a single gene and are derived from a precursor polypeptide by posttranslational cleavage. Beta-DG is an integral membrane protein, whereas alpha-DG is membrane-associated through its noncovalent interaction with the extracellular domain of beta-DG. The alpha- and beta-DGs provide important physical linkages between components of basement membranes and cytoplasmic proteins that bind to the actin cytoskeleton. Alpha-DG is a heavily glycosylated, mucin-like protein anchored on the extracellular surface of the myotube, where it may provide linkage between the sarcolemma and extracellular matrix (ECM). Alpha-DG is expressed in a variety of fetal and adult tissues. Tissue-specific glycosylation modifies the laminin specificity of alpha-DG. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alpha-DG has been shown to colocalize with laminin in skeletal and cardiac muscle and a number of other cells including peripheral nerve, astrocytes, Purkinje neurons and kidney epithelium. Laminin-10/11 was shown to bind preferentially to brain alpha-DG. In Duchenne muscular dystrophy, the expression of alpha-DG is dramatically reduced leading to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. In the central nervous system, dystroglycan functions as a dual receptor for agrin and laminin-2 for instance in the Schwann cell membrane. Furthermore, defects in dystroglycan are central to the pathogenesis of structural and functional brain abnormalities seen in congenital muscular dystrophies (CMD).Synonyms: DAG1, Dystrophin-associated glycoprotein 1

    Gene ID

    281439

    NCBI Accession

    NP_776587

    UniProt

    O18738

    Pathways

    Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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