PI3 antibody

Catalog No. ABIN1107080

The Mouse Monoclonal anti-PI3 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PI3 in samples from Human.

Quick Overview for PI3 antibody (ABIN1107080)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: TRAB2O

Product Details anti-PI3 Antibody

Specificity: TRAB2O recognises all known Trappins via the GQDPVK epitope. TRAB2O shows strong reaction with Human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa). For detection of fully processed Elafin/SKALP (C-terminal 57 aa) we advise to use AM26266PU-N. The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: Elafin / PI3

Background: Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria.Synonyms: ESI, Elastase-specific inhibitor, PI-3, Peptidase inhibitor 3, Protease inhibitor WAP3, SKALP, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, WAP3, WFDC14

Gene ID: 5266

NCBI Accession: NP_002629

UniProt: P19957