Laminin beta 1 antibody

Catalog No. ABIN1108020

This anti-Laminin beta 1 antibody is a Mouse Monoclonal antibody detecting Laminin beta 1 in WB, IHC (fro) and EIA. Suitable for Human.

Quick Overview for Laminin beta 1 antibody (ABIN1108020)

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Laminin beta 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: DG10

Product Details anti-Laminin beta 1 Antibody

Specificity: The antibody recognizes the Mr 220000 β1-chain of laminin. Clone DG10 appears to react with the NH2-terminus of the Ln β1 chain (1). The antibody reacts with Human tissues.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ig Fraction

Immunogen: Partially pepsin-digested laminins from Human placenta (ref.8). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1.0 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Laminin beta 1

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Alternative Name: Laminin beta-1 / LAMB1

Background: Laminin, a major structural glycoprotein of the basement membranes, is an attachment and survival factor for epithelial cells and can also promote the growth of epithelial cells. Laminin plays an important role in both the structural organization of basement membranes and in the anchorage of cells. The monoclonal antibody, DG10 and BC17 against laminin chain ß1 and γ1, respectively can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing these chains. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning, for instance, the integrity of the basement membranes in carcinomas. Clone DG10 against Laminin ß1-chain is apparently one of the few laminin antibodies working perfectly in immunoblotting, thus making it especially valuable for biochemical studies.Synonyms: Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta

Gene ID: 3912

NCBI Accession: NP_002282

UniProt: P07942