PRG2 antibody

Catalog No. ABIN110842

This Mouse Monoclonal antibody specifically detects PRG2 in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for PRG2 antibody (ABIN110842)

Target: PRG2 (Bone Marrow Proteoglycan (PRG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: BMK13

Product Details anti-PRG2 Antibody

Specificity: Specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as an eosinophil marker. The antibody will recognize the 23.8 kDa secreted pro-form of MBP (Pregnancy associated MBP) and the 13.8 kDa mature form found in the matrix of the eosinophil large specific granule. Does not cross-react with horse, mouse or rat MBP.

Purification: Protein A chromatography

Isotype: IgG1

Application Details

Application Notes: IHC with fresh frozen tissue (fix in methanol: acetone)IHC with formalin fixed paraffin-embedded tissue (treat with 0.1 % trypsin before blocking)Western blotELISA
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative and 0.1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for PRG2

Target: PRG2 (Bone Marrow Proteoglycan (PRG2))

Alternative Name: Proteoglycan 2

Background: PRG2 is a cytotoxin and helminthotoxin. It also induces noncytolytic histamine release from human basophils and is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.Synonyms: BMPG, Bone marrow proteoglycan, EMBP, Eosinophil granule major basic protein, PRG2, Pregnancy-associated major basic protein

Gene ID: 5553

NCBI Accession: NP_002719

UniProt: P13727