NOS1 antibody (N-Term)

Catalog No. ABIN1108474

This Mouse Monoclonal antibody specifically detects NOS1 in WB. It exhibits reactivity toward Human, Rat, Goat and Pig.

Quick Overview for NOS1 antibody (N-Term) (ABIN1108474)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Goat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: N1

Product Details anti-NOS1 Antibody

Binding Specificity: AA 1-181, N-Term

Specificity: This antibody reacts to NOS1 (1-181).

Purification: Affinity chromatography

Immunogen: Protein A fusion protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % sodium acetate or neutral PBS

Concentration: 0,1 mg/mL

Buffer: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background: Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect ofendothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2.Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.Synonyms: Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Nitric Oxide Synthase, bNOS, nNOS

Gene ID: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction