Sonic Hedgehog antibody (N-Term)

Catalog No. ABIN1109100

This anti-Sonic Hedgehog antibody is a Rabbit Polyclonal antibody detecting Sonic Hedgehog in WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Sonic Hedgehog antibody (N-Term) (ABIN1109100)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Sonic hedgehog (SHH) near N-term. No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence near the N-terminal of human SHH .Remarks: Sequence is identical to the related mouse and rat sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Background: The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined, however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.Synonyms: HHG-1, Sonic hedgehog protein

Gene ID: 6469

NCBI Accession: NP_000184

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development