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Sonic Hedgehog antibody (N-Term)

SHH Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Goat, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2781775
  • Target See all Sonic Hedgehog (SHH) Antibodies
    Sonic Hedgehog (SHH)
    Binding Specificity
    • 15
    • 10
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 61
    • 53
    • 39
    • 17
    • 8
    • 6
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Goat, Rabbit
    Host
    • 73
    • 10
    • 8
    • 1
    • 1
    Rabbit
    Clonality
    • 65
    • 28
    Polyclonal
    Conjugate
    • 53
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sonic Hedgehog antibody is un-conjugated
    Application
    • 73
    • 32
    • 24
    • 14
    • 13
    • 11
    • 11
    • 8
    • 7
    • 7
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Sequence
    RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT
    Predicted Reactivity
    Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
    Characteristics
    This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
    Top Product
    Discover our top product SHH Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 462 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    Sonic Hedgehog (SHH)
    Alternative Name
    SHH (SHH Products)
    Synonyms
    HHG1 antibody, HLP3 antibody, HPE3 antibody, MCOPCB5 antibody, SMMCI antibody, TPT antibody, TPTPS antibody, 9530036O11Rik antibody, Dsh antibody, Hhg1 antibody, Hx antibody, Hxl3 antibody, M100081 antibody, fc83d08 antibody, shh antibody, syu antibody, vhh-1 antibody, vhh1 antibody, wu:fc83d08 antibody, Xhh antibody, hedgehog antibody, xshh antibody, SHH antibody, twh antibody, twhh antibody, sonic hedgehog antibody, sonic hedgehog a antibody, sonic hedgehog L homeolog antibody, sonic hedgehog protein A antibody, sonic hedgehog b antibody, SHH antibody, Shh antibody, shha antibody, shh.L antibody, shh antibody, shhb antibody
    Background
    SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
    Alias Symbols: HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, MCOPCB5
    Protein Interaction Partner: UBC, SEL1L, DERL2, DERL1, SYVN1, HHIP, PTCH2, PTCH1, SHH, ADCYAP1, GAS1,
    Protein Size: 462
    Molecular Weight
    28 kDa
    Gene ID
    6469
    NCBI Accession
    NM_000193, NP_000184
    UniProt
    Q15465
    Pathways
    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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