TJP2 antibody (AA 411-467)

Catalog No. ABIN1109264

The Guinea Pig Polyclonal anti-TJP2 antibody has been validated for WB, IF and IHC (fro). It is suitable to detect TJP2 in samples from Human and Mouse.

Quick Overview for TJP2 antibody (AA 411-467) (ABIN1109264)

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reactivity: Human, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TJP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TJP2 Antibody

Binding Specificity: AA 411-467

Specificity: This antibody recognizes human zona occludens 2 (ZO-2).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Protein A

Immunogen: Amino acids 411-467 of human ZO-2

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, containing 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Alternative Name: TJP2

Background: Zona occludens 2 (ZO-2) is an ~160 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.Synonyms: Tight junction protein 2, Tight junction protein ZO-2, X104, ZO2, Zona occludens protein 2, Zonula occludens protein 2

Gene ID: 9414

NCBI Accession: NP_001164101

UniProt: Q9UDY2