Vinculin antibody

Catalog No. ABIN1109492

The Mouse Monoclonal anti-Vinculin antibody has been validated for WB, IHC (fro) and EIA. It is suitable to detect Vinculin in samples from Human.

Quick Overview for Vinculin antibody (ABIN1109492)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: FB11

Product Details anti-Vinculin Antibody

Specificity: The antibody reacts with the Vinculin antigen. In Immunoblotting the antibody identifies a 130 kDa polypeptide.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Purified Vinculin from Human smooth muscle. Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azdie, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Vinculin

Target: Vinculin (VCL)

Background: Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and alpha actinin, which in turn binds to actin. The binding of fibronectin is also indirect where vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor which itself is the ligand for fibronectin. Vinculin is widely distributed in tissue and is expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix.Synonyms: Metavinculin

Gene ID: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2