Vinculin antibody (AA 174-322)

Catalog No. ABIN6940864

The Mouse Monoclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human. It has been validated for WB and IHC.

Quick Overview for Vinculin antibody (AA 174-322) (ABIN6940864)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: VCL-2572

Product Details anti-Vinculin Antibody

Binding Specificity: AA 174-322

Purpose: Vinculin (Marker of Age-related Macular Degeneration) Antibody

Specificity: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: U-87, THP-1 cells Human Bladder and Testis.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL

Background:

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC

THP-1 cells Human Bladder and Testis., U-87

Molecular Weight: 117kDa

Gene ID: 7414, 643896

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2