ZBTB4 antibody

Catalog No. ABIN1109539

This Rabbit Polyclonal antibody specifically detects ZBTB4 in WB, IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for ZBTB4 antibody (ABIN1109539)

Target: ZBTB4 (Zinc Finger and BTB Domain Containing 4 (ZBTB4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-ZBTB4 Antibody

Specificity: This antibody reacts with 105 kDa ZBTB4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide derived from an internal domain of Human ZBTB4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in distilled water.

Buffer: 0.1 M Tris, 0.1 M Glycine, 2 % Sucrose, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ZBTB4

Target: ZBTB4 (Zinc Finger and BTB Domain Containing 4 (ZBTB4))

Alternative Name: ZBTB4

Background: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions, this degradation increases with DNA damage.Synonyms: KAISO-L1, KAISO-like zinc finger protein 1, KIAA1538, Zinc finger and BTB domain-containing protein 4

Gene ID: 57659

NCBI Accession: NP_001122305