Triiodothyronine T3 antibody

Catalog No. ABIN111010

Product Details anti-Triiodothyronine T3 Antibody

Target: Triiodothyronine T3 (T3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Triiodothyronine T3 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Purification: Protein A chromatography and 0.22 µm filteration

Clone: ME-124

Isotype: IgG1

Application Details

Application Notes: Works in ELISA. The antibody can be used as a capture or tracer antibody in enzyme-Immunoassays(See ''Protocols'').
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: As a capture, the antibody should be immobilized at a concentration of 3 µg/ml in 0. 2 Mcarbonate/bicarbonate buffer, use 50-100 µl/well to coat. As a tracer, the antibody can be conjugated to horseradish peroxidase (HRP) or biotin as ameans of elaborating the specific reaction.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (OD280 nm, E0.1% = 1.36)

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Alternative Name: Triiodothyronine / T3 (T3 Products)

Target Type: Amino Acid

Background: Triiodothyronine is a hormone made by the thyroid gland. It has three iodine molecules attached to its molecular structure. It is the most powerful thyroid hormone, and affects almost every process in the body, including body temperature, growth, and heart rate.