APOA1 antibody

Catalog No. ABIN111087

The Mouse Monoclonal anti-APOA1 antibody (Clone 1404) (ABIN111087) specifically detects APOA1 in RIA and EIA. The antibody is reactive with Human samples.

Quick Overview for APOA1 antibody (ABIN111087)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: 1404

Product Details anti-APOA1 Antibody

Specificity: Hihgly specific for APO A-I, no cross reactivity with APO A-II or Apo B.Aff. Const.:8 x 10e9

Purification: DEAE-chromatography

Immunogen: Purified human plasma Apo A-I

Isotype: IgG1

Application Details

Application Notes: Competeitive RIA. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 3.84 mg/mL (OD280 nm, E1% = 14)

Buffer: 0.015 M PBS, 0.15 M NaCl, pH 7.2 with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apolipoprotein A I (APO AI)

Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gene ID: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism