ALPL antibody

Catalog No. ABIN111088

This Mouse Monoclonal antibody specifically detects ALPL in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for ALPL antibody (ABIN111088)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: BGN-03-661 (BG-03-661)

Product Details anti-ALPL Antibody

Specificity: The antibody is specific for Alkaline Phosphatase. It detects the tissue specific isotypes, BAP, PLAP, and IAP (Bone, Placental and Intestinal AP), but may react with other Alkaline Phosphatases.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: Alkaline Phosphatase prepared from Human bone.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: In Immunoblotting this antibody stains a band of BAP at around 70 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline Phosphatase / ALPL

Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: AP-TNAP, Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, TNSALP, tissue-nonspecific isozyme

Gene ID: 249

NCBI Accession: NP_000469

UniProt: P05186