CD63 antibody (Biotin)

Catalog No. ABIN7566513

The Mouse Monoclonal anti-CD63 antibody has been validated for FACS and IF. It is suitable to detect CD63 in samples from Human.

Quick Overview for CD63 antibody (Biotin) (ABIN7566513)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TEA3-18

Product Details anti-CD63 Antibody (Biotin)

Characteristics: Monoclonal Mouse Anti-Human CD63 BIOTIN is recommended for use in flow cytometry for identification of activated platelets.

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 µl, 1/100, of the suggested working dilution to label 10^6 cells.

Comment:

BIOTIN (Sigma).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. CD63 contains four hydrophobic transmembrane domains with a major extracellular region of 95 amino acids between transmembrane segments 3 and 4. The COOH-terminal sequence SGYEVM functions as a lysosomal targeting sequence. This molecule is also referred highly to as LIMP, gp55,melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). It is also widely expressed on surface and in the cytoplasm of various hematopoietic (monocytes, macrophages) and non-hematopoietic cells (endothelium, fibroblasts, osteoclasts, smooth muscle). Its cellular function has not been fully elucidated. Reports suggest this as a useful molecule to study platelet activation.