CD9 antibody

Catalog No. ABIN111240

This anti-CD9 antibody is a Mouse Monoclonal antibody detecting CD9 in FACS, IF and IHC (fro). Suitable for Human.

Quick Overview for CD9 antibody (ABIN111240)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ALB6

Product Details anti-CD9 Antibody

Specificity: The molecular weight of the recognized antigen is 24 kDa. This antibody recognizes 22-43 % of peripheral non-T cells (B cells) (4). Recognizes blood platelets and a few of granulous cells on normal bone marrow. Recognizes the majority of acute lymphoid leukemia, non-T, non-B, CALLA positive, some atypic or myeloid leukemias and chronic lymphoid leukemias. Recognizes the distal convoluted tubule and the distal right tubule of nephronds.

Immunogen: Human bone marrow malignant cells from acute non-T, non-B leukemia.

Isotype: IgG1

Application Details

Application Notes: Fluorescent Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry on Frozen Sections or Cell smears: 1/25-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1m l distilled water.

Concentration: 0.2 mg/mL (prior lyophilization)

Buffer: Lyophilized from PBS containing 1 mg/mL BSA as stabilizer without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 4 - 8 °C for one month or at -20 °C for longer.

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization