CD35 antibody

Catalog No. ABIN111245

The Mouse Monoclonal anti-CD35 antibody has been validated for FACS, IHC (p), IHC (fro) and Func. It is suitable to detect CD35 in samples from Human. There are 2+ publications available.

Quick Overview for CD35 antibody (ABIN111245)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: J3D3

Product Details anti-CD35 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: Purified C3b receptor from Human red blood cells.

Isotype: IgG1

Application Details

Application Notes: Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3bdependent rosette formation with Lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and onBouin's fixed, Paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL of distilled water.

Concentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS containing 1 mg/mL BSA as stabilizer and no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

References for anti-CD35 antibody (ABIN111245)

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Molnar: "Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of [14C]glucosamine and [14C]sialic acid into membrane proteins." in: Biochemistry, Vol. 6, Issue 10, pp. 3064-76 (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 / C3BR

Background: This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1

Gene ID: 9606

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity