PRKDC antibody (Catalytic Subunit)

Catalog No. ABIN1112714

The Mouse Monoclonal anti-PRKDC antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect PRKDC in samples from Mouse.

Quick Overview for PRKDC antibody (Catalytic Subunit) (ABIN1112714)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: N9

Product Details anti-PRKDC Antibody

Binding Specificity: Catalytic Subunit

Immunogen: Recombinant DNA-PKcs protein.

Isotype: IgG2a

Application Details

Application Notes: WB (1:1000), IHC-P (1:200), ICC (1:100). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Background: DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Anderson and Lees-Miller (1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. It acts as a molecular sensor for DNA damage, and it may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response