BMI1 antibody

Catalog No. ABIN1112825

The Mouse Monoclonal anti-BMI1 antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect BMI1 in samples from Mouse.

Quick Overview for BMI1 antibody (ABIN1112825)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: P9

Product Details anti-BMI1 Antibody

Immunogen: Recombinant Polycomb complex protein BMI-1 protein.

Isotype: IgG2a

Application Details

Application Notes: WB (1:1000-5000), IHC-P (1:200), ICC (1:500). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: Polycomb complex protein BMI-1

Background: BMI1 has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. It is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. BMI1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. BMI1 is also thought to inhibit ageing in neurons through the suppression of p53.

Pathways: Cell Division Cycle, Autophagy