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MCAM antibody

The Rabbit Polyclonal anti-MCAM antibody has been validated for FACS, WB and ICC. It is suitable to detect MCAM in samples from Mouse and Rat.
Catalog No. ABIN1112867

Quick Overview for MCAM antibody (ABIN1112867)

Target

See all MCAM Antibodies
MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity

  • 175
  • 54
  • 53
  • 15
  • 15
  • 8
  • 8
  • 6
  • 5
  • 2
Mouse, Rat

Host

  • 107
  • 102
  • 2
  • 1
  • 1
  • 1
Rabbit

Clonality

  • 116
  • 98
Polyclonal

Conjugate

  • 97
  • 17
  • 14
  • 9
  • 8
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MCAM antibody is un-conjugated

Application

  • 111
  • 97
  • 78
  • 42
  • 33
  • 30
  • 28
  • 23
  • 12
  • 12
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC)
  • Purification

    affinity purified

    Isotype

    IgG
  • Application Notes

    WB (1:1000-2000), ICC (1:100-500), Flow Cyt (1:10-100). Other applications have not been tested. The optimal dilutions should be determined by end user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

    Preservative

    Sodium azide, Thimerosal (Merthiolate)

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.

    Expiry Date

    12 months
  • Target

    MCAM (Melanoma Cell Adhesion Molecule (MCAM))

    Alternative Name

    CD146

    Background

    CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. 1 CD146 belongs to the immunoglobulin superfamily and expressed in all types of human endothelial cells. The regulated expression of CD146 at areas of cell-cell junction and strongly suggest involvement of CD146 as a mediator of cell-cell interaction. 2 CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, 3 and its expression may be linked to multipotency, mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface. 4
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