beta Actin antibody

Catalog No. ABIN1113002

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB, IHC, IF and IP. It is suitable to detect beta Actin in samples from Mouse, Rat, Chicken, Rabbit, Dog, Pig, Cow, Sheep, Guinea Pig, Cat, Leech and Carp.

Quick Overview for beta Actin antibody (ABIN1113002)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Mouse, Rat, Chicken, Rabbit, Dog, Pig, Cow, Sheep, Guinea Pig, Cat, Leech, Carp

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-beta Actin Antibody

Specificity: Detect the epitope located on N-terminal of beta-Actin.

Immunogen: Gizzard Actin of chicken origin.

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:100-1000), IF (1:50-500) ,IHC (1:50-500) . Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year. Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background: Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo. 1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets. 2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction